ISOLATION OF SCLEROGLUCAN ORIGINATED FROM SCLEROTINIA SCLEROTIORUM AND INVESTIGATION OF ITS BIOLOGICAL PROPERTIES

Background and Aim:Scleroglucan –the polymer of glucose with (β 1-3) glycoside bonds and (β 1-6) branches- is produced by the pathogenic fungus, Sclerotinia sclerotiorum. Considering chemical properties of scleroglucan, it has different applications in various industries such as petroleum, cosmetics, food and drug.Methods:In order to study immunomodulatory function of scleroglucan, it was isolated from S. sclerotiorum and its antibacterial and antioxidant properties was evaluated using disk diffusion agar, 2,2-Diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging test and Reactive Oxygen Species (ROS) assay. The structure of extracted scleroglucan was confirmed by Fourier Transform InfraRed (FTIR) spectroscopy and High Performance Liquid Chromatography (HPLC).Results: Under in vitro condition, no antibacterial activity was observed for extracted scleroglucan against Escherichia coli and Staphylococcus aureus. No toxic effect was detected on the normal healthy fibroblasts using cell viability (MTT=3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) test. DPPH experiment indicated that the antioxidant property of scleroglucan is up to 23 percent. ROS test showed that the florescent intensity in 1000 ug.ml-1 concentration of extract decreased significantly.Conclusion:MTT assay proved that scleroglucan has no cytotoxic effect on normal fibroblasts. Antioxidant tests (DPPH and ROS) showed that scleroglucan could be a antioxidant agent against free radicals but in the disk diffusion agar no direct antibactrial effect was shown.

کلیدواژه ها:

antibacterial ، antioxidant effect ، Isolation ، scleroglucan ، sclerotinia sclerotiorum