THE EFFECT OF SILICON DIOXIDE AND ZEOLITE-ZINC NANOPARTICLES AGAINST STREPTOCOCCUS MUTANS BIOFILMS

Background and Aim:Streptococcus mutans are able to form biofilms on the surface of oral cavity. The pathogenicity of Streptococcus mutans is related to the production and tolerance of acid. Silicone and zeolite nanoparticles(NPs), due to porous structure, high levels of absorption and antimicrobial properties in the oral cavity, reduce the spread of biofilms at the surface of the teeth.Methods:Standard strain of Streptococcus mutans, Silicon dioxide and zeolite-zinc NPs from the Tehran University of Medical Sciences Research Center were prepared. NPs with 2 mg / ml concentration diluted into Tryptic Soy Broth and Todd hewiett broth by the addition of bacteria and incubation for 24 hours, 5% CO2at 37 °C. The minimal inhibitory concentration (MIC) was studied based on the opacity and lack of it.To determine MBC , Transfer 10 μL of the last well with opacity to Blood Agar culture and incubation for 24 hours, CO2 and 37 ° C, and MBC were studies based on colony formation.Results:MIC for Streptococcus mutans in NPs of silicon dioxide with CO2 and without CO2 were 2 mg / ml and 1 mgr/ml, respectively. Minimum Bactericidal Concentration) MBC( was 4 mg/ml and 2mgr/ml. MIC for Streptococcus mutans in zeolite-zinc NPs under conditions of CO2 and without CO2 were 32 mg/ml and 8mg/ml, respectively, and MBC was 64 mg/ml and 8 mg/ml, respectively.Conclusion:The study showed that silicon dioxide and zeolite-zinc NPs are an effective inhibitor against the formation of biofilms and dental plaque from Streptococcus mutans.

کلیدواژه ها:

nanoparticles ، silicon dioxide ، zeolite ، biofilm ، Streptococcus mutans ، dental plaque