ISOLATION AND SUDY OF DRUG-RESISTANT GENE (EFFLUX PUMP) IN PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL SAMPLES AND MILK FOOD BY MPCR AND DETERMINE ANTIBIOTIC SUSCEPTIBILITY PATTERN

Background and Aim:Pseudomonas aeruginosa is an important cause of nosocomial infections in burn patients, one of the most important types of bacteria resistant to antibiotics and therefore aeruginosa infection is difficult to treat. Efflux pumps mex key role in the development of multiple resistance to other antimicrobial drugs. OprM-MexAB pump capable of a range of antimicrobial compounds without any similarity between the structure and function will leak out of the cell. This study aimed to investigate prevalence of isolated genes of efflux pumps mex and the Determination of antibiotic resistance on ceftriaxone,clavonic acid,gentamicin and amikacin was performed.Methods:Initially 30 samples of P. aeruginosa isolated from clinical specimen’s burn wounds Tehran hospitals and 30 food samples of milk, after approval by the diagnostic tests Vaftraqy, were studied. Finally, for every 60 samples, isolated, Multiplex PCR was performed to detect target genes to be considered as the gold standard in the sense that its results are more reliableResults:According to the results obtained using the PCR gene efflux pumps has been mex 100℅ and The highest resistance to antibiotic ceftriaxone was observed in comparison with amicassin.Conclusion:Mentioned results correspond with the results of other studies and Due to the presence of 100% of the genes of the pump of Influx in both clinical specimens and infected milk of Pseudomonas aeruginosa and due to the chromosomal nature of these genes, they can be used as markers for the identification of Pseudomonas aeruginosa by molecular methods.

کلیدواژه ها:

Psudomonas aeroginosa ، Multiplex PCR ، Drug resistans