COMPARISON OF GENOMIC POLYMORPHISM OF ENTEROCOCCUS FAECALIS ISOLATED FROM CLINICAL SAMPLES USING ERIC-PCR AND BOX-PCR METHODS

Background and Aim:Enterococci are gram positive and catalase-negative cocci. The most common Enterococci involved in human infection is Enterococcus faecalis, which consists of 85-90% of cases. Several molecular methods are used for bacterial genotyping. Various methods have been used for the typing of these bacteria, including polymerase chain reaction based on Repetitive-Element PCR (rep-PCR) and BOX-PCR.Methods:In the cross-sectional study, 60 clinical isolates of E. faecalis were obtained from patients with urinary tract infection referred to Shariati Hospital from a 6-month period. After confirming of E. faecalis strains using standard biochemical and microbiological methods, genomic DNA was extracted using a DNA extraction kit (Fermentas, UK). Then genomic polymorphism was performed using ERIC-PCR and BOX-PCR. Finally, using the NTsys software, the phylogeny tree was mapped.Results:phylogenetic tree showed that all strains had 25 distinct clusters at a level of similarity of 58%. The highest number of strains was found in ERIC-PCR in the fifteenth group, the nineteen isolates and in the BOX-PCR group in the seventeenth and eighteenth groups, six isolates. The power of BOX-PCR and ERIC-PCR differentiation was 0.95% and 85%, respectively.Conclusion:In the present study, E. faecalis strains have different BOX patterns that indicate the genetic diversity of E. faecalis strains, and probably these strains have a different origin that is spinning between animals and humans.

کلیدواژه ها:

Enterococcus faecalis ، BOX-PCR ، ERIC-PCR