CHARACHTRIZATION OF ASAIA SP. ISOLATED FROM MALARIA VECTORS IN SOUTHWEST OF IRAN

Background and Aim:different strategies have been suggested by scientists to control the malaria disease. Paratransgenesis is one of the solutions for vector control. In this strategy, symbiont microorganisms are used for disrupting the parasite life cycle or vector fitness. Asaia is a bacterium from acid acetic alpha proteobacterium which stably associated with some mosquito species like several Anopheles. Asaia has some specific characteristics that make it as a potent candidate for paratransgenesis to control malaria such as: cultivation in cell-free media, colonization in different parts of the vector body (gut, salivary glands, reproductive organs, etc.), easy transformation and vertical and horizontal transmission to larvaeMethods:Different larval smples of Anopheles were collected from Fars province of Iran. Samples were transfered to Insectary of school of health. Bacteria were isolated from larval and adult midguts and cultured in specefic media of Asaia. Morphological and biochemical analysis were performed on each sample. Molecular confirmation was performed by 16s rRNA PCR with genus specific primers.Results:Based on the specific culture situations and molecular test, we isolated Asaia bacteria from An. Stephensi, An.fluvitilis and An.D,thali. the samples collected from kazeron and nour-abad districts in summer2016Conclusion:Asaia has some basic characteristics such as: no pathogenicity for human, simple genetic manipulating, vertical and horizontal transmission, cultur on non-expensive media. Therefore, Asaia can be considered as a very potent agent for developing a robust paratransgenesis tool to control malaria based on vector control.

کلیدواژه ها:

Asaia ، Malaria ، paratransgenesis ، Anopheles ، 16s rRNA