APPROPRIATE CIRCADIAN DARKNESS MAY HELP RECOVERY OF SEPTIC SHOCK PRODUCED BY BACTERIAL LIPOPOLYSACCHARIDE

Background and Aim:Sepsis is an important killing form of bacterial infection in burned patients. They may suffered Pseudomonas aeroginusa colonization in their lesions and toxicate with its lipopolysaccharide (LPS) systematically which result in cell stress and activation of unfolded protein response (UPR) in different tissues like liver. In another hand circadian condition capability to modified endogenous hormones like melatonin confirmed previously.Methods:P. aeroginusa bacteria were isolated from the burned hospitalized patients. After bacterial identification, their LPS purified by specific LPS extraction Kit and administrated to LPS treated study group between fifty six male C57BL6 mice which kept in 2 and 6 hours in light and dark. Serum of animals in study groups check for the level of melatonin and simultaneously liver of animals in study groups removed after surgery and their RNA extracted to investigate by RT-PCR for activation of specific UPR mRNA X-Box Binding Protein-1 (XBP-1) gene.Results:Mice which kept 6 hours in dark show the most elevated serum level of melatonin and present significant unspliced pattern in xbp-1 mRNA gene.Conclusion:Our data revealed clearly preparing appropriate circadian darkness result in natural secretion of melatonin and decreased level of xbp-1 mRNA splicing.

کلیدواژه ها:

Pseudomonas aeroginusa ، Burned patients ، LPS ، UPR ، XBP-1 ، Melatonin