DETECTION OF FOWL ADENOVIRUS E IN BROILER FLOCK IN GOLESTAN PROVINCE , 2018

Background and Aim:Inclusion body hepatitis (IBH) associated with FAdV-I ( fowl adenovirus) occurs worldwide in poultry industry. It is an acute disease that mainly affects young chickens between 3 and 7 weeks of age, and is caused by several avian adenovirus serotypes. At histopathological ex-amination, the organs with the most significant lesions were the liver, intestines and lungs.Methods:The samples were obtained from 2 weeks old broiler flock of Ross from Golestan province of Iran in March 2018. The samples (liver) were homogenized and extracted by Sina Pure DNA kit according to the protocol of the manufacturer. The 590 bp region of the hexon gene was amplified using a pair of specific primers. The reaction product were analyzed by electropho-resis. The RT-PCR product sequenced in the forward and reverse direction.Results:The results indicated that two FAdV serotypes (11 and 8b) are high prevalence serotypes of FAdVs in Iran and are pathogenic to cause IBH in young chicks. The nucleotide sequences of hexon genes were compared with the FAdV sequences data available in the GenBank. This isolate is located in genotype E clade.Conclusion:This is the first report of FADV in broiler farms in Golestan province. It is necessary to provide more epidemiological data to establish the prevalence of these viruses and justify the development of vaccine programs in breeders, broiler and laying hens to prevent vertical and horizontal virus infections and prevent the spread of this virus.

کلیدواژه ها:

Inclusion body hepatitis ، Adenovirus ، FADV