ISOLATION, IDENTIFICATION AND OPTIMIZATION OF MICROBIAL OIL PRODUCTION AND EVALUATION OF THE ANTIBACTERIAL EFFECT
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 392
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شناسه ملی سند علمی:
MEDISM19_387
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Oleaginous yeasts have attracted much attention because of being single cells, lack of endotoxin and the possibility of large-scale biotechnological production. Oleaginous yeasts that are classified as GRAS (safety microorganisms) are suitable for single cell oil productionMethods:Evaluating of fatty acid profiles before and after optimization process using gas chromatography-mass spectrometry and finally evaluation of its antibacterial properties, were conducted. The best yeast strain was identified as Cryptococcus heimaeyensis using PCR method. After optimization of lipid production with Taguchi method, lipid production and lipid yield were reached to 8.05 g/L and 58.33%, respectivel. Esterified lipid composition contains linoleic acid, oleic acid and palmitic acid, as the most fatty acids in the fatty acid profile of the yeast. Oil extractions from yeasts have very similar fatty acid profile to vegetable oils and showed antimicrobial effect.Results: Isolation, identification and optimization of microbial oil production and evaluation of the antibacterial effect was studied in this studyConclusion:: Finding of native strains with high potential of lipid production and optimization through experimental design method is of great importance in the fields of biotechnological productions
کلیدواژه ها:
نویسندگان
Fatemeh Khosravani por
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Mahboobeh Madani
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Marjan Enshaeieh
Department of biotechnology,Iranian Reasearch Organization for science andTechnology (IROST),Tehran,Iran