EXPRESSION OF RECOMBINANT P48 PROTEIN OF MYCOPLASMA BOVIS IN E. COLI, APPLICABLE TO ELISA TEST

Background and Aim:Mcoplasma bovis causes a range of diseases in cattle throughout of the world including arthritis, pneumonia and mastitis. Early diagnosis of Mcoplasma bovis infection is important to effective treatment and control of disease.Methods:In first step, all of nucleotide and protein sequences of p48 were collected from GenBank. The pattern of Iranian p48 protein were compare with available sequences from other regions. Based on bioinformatics tools a specific protein sequences was selected with high population coverage in Iran. For suitable expression in E.coli, all Codons were optimized in complete coding target sequence. Complete coding sequences of P48 protein was cloned to pET28a+ vector. Expression of recombinant P48 protein was optimized in Bl21 strain of E. coli.Results:Results showed that the recombinant p48 protein of mycoplasma bovis was expressed in Bl21 strain of Ecoli successfully.Conclusion:In this study, we attempt to design a construct for expression of recombinant P48 protein of Mycoplasma bovis as an antigen for application in diagnostic elisa test.