SCALE UP OF RHAMNOLIPID-TYPE BIOSURFACTANT PRODUCTION BY PSEUDOMONAS AERUGINOSA MA01 IN A 5-L BIOREACTOR

Background and Aim:We report here the fermentative production of a rhamolipid-type biosurfactant by Pseudomonas aeruginosa MA01 from shake flasks to a bench-scale bioreactor using sunflower oil as a sole source of carbon at 30°C. Rhamnolipid biosurfactant retained its properties including surface activity, CMC, lower toxicity, high foaming, and etc. throughout fermentation in a 5-L bioreactor.Methods:The conditions under which the study was set entailed agitation speed of 300 rpm, aeration rate 1.5 vvm, pH 7, temperature 30°C, and sunflower oil 3.5%.Results:When the bacterium reached its maximum growth extent, it began producing rhamnolipid biosurfactant under shaking after a process time of 168 hours.Conclusion:The highest amount of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa was found to be 40 g/l which was more than 38% of the shake flask.

کلیدواژه ها:

Biosurfactant ، Rhamnolipid ، Pseudomonas aeruginosa…. ، Scale-up ، Bioreactor