PRODUCTION OF BACTERIAL CELLULOSE USING SUGARCANE VINASSE

Background and Aim:In comparison with chemically synthesized polymers, biopolymers due to their physicochemical properties have been paid attention Cellulose is one of the most applied polymers that has a broad range of applications from High-strength paper and diet foods production to medical applications such as soft tissue replacement and artificial blood vessels production. The aim of this study was to use sugarcane vinasse as low cost and available substrate for cellulose production.Methods:For this purpose Gluconoacetobacter xylinus PTCC 1734, the type species for cellulose production was inoculated in to sterile pure as well as 5 and 10 % concentrations of vinasse and incubated at both static and shaking conditionsat 30°C for 8 days. Simultaneously, hestrin schramm medium was used with same incubation conditions. The produced cellulose was washed by distilled water and treated with 0.1M NaOH. Cellulose production was confirmed according molish and benedict tests and the dry weight of cellulose was calculated.Results:As a result the highest cellulose production was achieved at pure vinasse in static condition as 0.201 g while in case of hestrin schramm the cellulose yield was0.097 g.Conclusion:These results suggest that vinasse which is a waste byproduct of sugarcane industry have the potential to be used as substrate for cellulose biopolymer production. It will reduce production costs and hence promote commercial production of this biopolymer.

کلیدواژه ها:

biopolymers ، bacterial cellulose ، G.Xylinus ، sugarcane ، vinnase