: DETERMINATION OF ANTIBIOTIC RESISTANCE PATTERN AND THE PREVALENCE OF BETA-LACTAMASE GENE BLATEM, SHV AND DHA IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 571

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شناسه ملی سند علمی:

MEDISM19_195

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:The presence of ESBL genes play an important role in resistance to beta-lactam antibiotics producing strains of these enzymes play . Gram-negative bacteria including Pseudomonas aeruginosa resistance to various antibiotics , beta-lactams and carbapenems especially increasingly been reported . This study aimed to investigate the prevalence of beta-lactamase genes in Pseudomonas aeruginosa isolates was performed by PCR method.Methods:103 strains of Pseudomonas aeruginosa isolated from clinical samples of urine, sputum, burn wounds and devices dialysis pediatric hospitals in the cities of Qom and Tehran, with the Gram stain, a test of biochemical differentiation and PCR District 16s rRNA detection were identified. Resistance to Ceftazidime/clavulanic acid and cefotaxime/ clavulanic acid 10 other common antibiotics was determined by disk diffusion method. Genomic DNA was extracted by method kit was performed and the beta-lactamase gene amplification with primers Amplicon available and were designed and genes were analyzed by PCR.Results:Of the total 103 isolates of Pseudomonas aeruginosa, 50 isolates with broad-spectrum beta-lactamase phenotype were observed. 46 isolates (47/38%) were carriers of TEM-1 beta-lactamase gene. 35 isolates had SHV gene and none of the isolates was observed in DHA gene.Conclusion:Pseudomonas aeruginosa beta-lactamase largely resistant to other common antibiotics and to select a suitable drug for destroying their antibiotic susceptibility testing should be done.

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نویسندگان

Reza Yari

Assistant Professor of Cell Biology, Department of Biology, Faculty of Sciences, Borujerd Branch, Iran

Mohammad Torkashvand

MSc. Student, Department of Biology, Faculty of sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

Mohammad Reza Mehrabi

Department of Laboratory Sciences, Medical Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran