EVALUATING THE SIMULTANEOUS IMPACT OF NANOPARTICLES WITH NETTLE EXTRACT AGAINST KLEBSIELLA THROUGH A CHECKERBOARD METHOD

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 477

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شناسه ملی سند علمی:

MEDISM19_146

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Klebsiella pneumonia is seen numerously in people hospitalized at the hospitals as well as people with weak immunity, so antibiotic resistance is a serious threat in this bacteria. Silver nanoparticles and many herbal extracts influence a wide range of bacteria due to the large antibacterial effect. The purpose of this study is to investigate the simultaneous effect of silver nanoparticles with nettle extract against Klebsiella bacteria through a checkerboard method.Methods:350 samples were collected from patients hospitalized in all wards of Rasool-e-Akram hospital. 112 Klebsiella strains were isolated using biochemical tests. Among them, resistant isolates were separated. The effects of silver nanoparticles, nettle extract, the combination of nettle extract and silver nanoparticles on the isolated bacteria were investigated using MIC and checkerboard methods.Results:The results showed that MIC for nettle extract is 0/7mg/ml against both isolates and is 0/78mg/ml for silver nanoparticles against ATCC isolate and is 3/125 mg/ml against MDR isolate. Simultaneous application of silver nanoparticles and nettle extract has a synergistic effect against the resistant isolate and an incremental effect against the standard isolate.Conclusion:Due to the increasing number of hospital infections and because bacteria are resistant against existing antibiotics, it is important to find a substitute for antibiotics with new materials having antibiotic characteristic, in near future. Because their production is cost effective and they have a good effect on resistant bacteria, they can be used in antibiotic treatments and their antibacterial effect can be exploited.

نویسندگان

Mahsa Dadgar

Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran

Sara Minaeian

Antimicrobial Resistance Research Center, Institute of Immunology and Infectious Diseases, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran

Somayeh Samimi

Ebnemasouyeh pharmaceutical company

Meisam Sadeghpour Karimi

Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran