PHYLOGENETIC ANALYSIS AND ANTIBIOTIC PATTERN DETERMINATION OF ESCHERICHIA COLI STRAINS ISOLATED FROM THE COAST OF THE GENAVEH REGION

Background and Aim:Escherichia coli belong to the group of bacteria with the name of coliform which is member of Entrobacteriacea family.Escherichia coli is as an important organism which could disseminate antibiotic resistancy in water shoreline. Accordingly, the present study tried to isolate the bacterium from Genaveh shoreline and sediments. Then phylogenetically chategorized based on Clermont technique and evluate their antibiotic and enzymatic pattern.Methods:In the present study 60 samples ( 30 water and 30 sediment) were collected from water and sediments between april to agust 2016. Then they were evaluated by MPN technique and serially dilutied samples were cultivated on MacConkey and Eosin methylene blue agar. After that they were identified using Gram stain, Catalase and oxidase tests. The phylogentical analyasis of E.coli were done using primers: ChuA, yjaA, TSPE 4.C2 and enzymatic pattern were evaluated usin APizyme kitResults:Out of 60 collected samples, 13 samples were E.coli, 9 from water and 4 from sediments. Based on antibiogram were resistancy to Ampicillin were 54% while and the sensitive pattern belong to Gentamicin (100%) and Ceftriaxone (92%). The most common phylogenic group were A, B2 and D. Although most of them belong to group D and the group have most of the virulent genes therefore, they had most of enzymes which is not common for other strains. Based on our finding human and animal commensal group with less enzymes had different enzyamatic pattern with group DConclusion:Overall, the best antibiotic for treatment on the same isolates is gentamicin and ceftriaxone which could be use in this geographical area.

کلیدواژه ها:

Escherichia coli ، Antibiotic pattern ، Enzymatic pattern ، Triplex PCR ، Genaveh shoreline