IDENTIFICATION OF BLACTX-M, BLASHV, AND BLATEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD

Background and Aim:Pseudomonas aeruginosa is one of the most important causes of opportunistic infections. The infection caused by P. aeruginosa is commonly severe and life-threatening and hard to treat due to limited susceptibility to antibiotics and development of resistance during treatment. The aim of the present study was to identify blaCTX-M, blaSHV, and blaTEM genes in P. aeruginosa strains isolated from human and animal samples using Multiplex-PCR method.Methods:In this descriptive cross-sectional study, a total of 120 isolates of P. aeruginosa were obtained from of human samples and animal raw milk. Antibiotic susceptibility test was determined by gel diffusion method and according to CLSI guidelines. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram-negative Bacteria and CSF) and Multiplex-PCR was performed for identification of blaCTX-M, blaSHV, and blaTEM genes.Results:In this study, the highest resistance in human and animal samples, was seen to amoxicillin and amikacin (100%) and the lowest resistance was to ciprofloxacin with frequency of 90% in human samples and 93.4% in animal samples. Based on the CDT results, 37(60.8%) isolates showed ESBLs phenotype, and based on the M-PCR results, 26 (21.6%) and 41 (34.2%) strains had blaCTX-M and blaTEM genes, respectively.Conclusion:The results of this study indicated that P. aeruginosa strains have high levels of resistance. Also, the frequency of these genes is high among human and animal isolates, therefore controlling these bacteria in humans and animals is of particular importance.

کلیدواژه ها:

Pseudomonas aeruginosa ، Beta-lactamase ، Antibiotic susceptibility ، Drug hypersensitivity