MOLECULAR ANALYSIS OF GENES OF ESBL )SHV.TEM.CTX) IN SHIGELLA SONNEI ISOLATED FROM CLINICAL SAMPLES BY PCR

Background and Aim:Shigella is one of the most common causes of dysentery and sometimes mortality specially in children and immunocopromised patients and the occurrence of drug resistance, makes it difficult the choice of appropriate antibiotics to treat shigellosis. The aim of this study was to define the prevalence of blaCTX-M, blaTEM and blaSHV in Shigella Sonnei isolated from patients with diarrhea by Multiplex-PCR.Methods:In this cross-sectional study, a total of 60 Shigella Sonnei isolates of private laboratories and hospitals in Northern Tehran different ages from diarrhea samples were collected and cultured in specific environments and with biochemical and serological confirmation. To identify resistan strains t to beta test was conducted phenotypic combine disk. The presence of blaCTX-M, blaTEM and blaSHV genes was evaluated by specific primers and Multiplex-PCR.Results:The highest frequency resistance to the antibiotics penicillin and erythromycin was 96.6% and 95%, respectively, and prevalence maximum sensitivity has been reported to ciprofloxacin and imipenem with 70%. In the Combine disk method, 29 strains (48.3%) were found positive to beta-lactamase. Among 60 isolates, 39 samples (65%) had the blaTEM gene, 28 samples (46.6%) had the blaCTX-M gene and 1 sample (1.66%) had the blaSHV gene.Conclusion:: Given the high prevalence of beta-lactam antibiotics resistance genes in strains of Shigella Sonnei , careful medical care and proper use of antibiotics appropriate is essential and timely to prevent the spread of resistant strains is necessary.

کلیدواژه ها:

ESBL ، Shigella Sonnei ، Multiplex-PCR