CHARACTERIZATION OF SHEEP AND GOAT VACCINE STRAINS OF MYCOPLASMA AGALACTIAE FROM RAZI INSTITUTE BY MOLECULAR TYPING ANALYSIS

Background and Aim:Agalactia is a contagious disease in small ruminants. In 1968 the sheep and goat strains of M. agalactiae were collected respectively from Iranian diseased animals. These strains have been used ever since for preparation of Contagious agalactia vaccine at Razi institute. Multi Locus Sequence Typing (MLST) and multiple locus variable number of tandem repeat analysis (MLVA) analysis are standard methods of genotyping for M.agalactiae. This is was conducted to assess genomic structure of the vaccine M. agalactiae strains at Razi institute.Methods:In the present study, Genes were chose for MLST and MLVA based on the sequenced genome of M.agalactiae type strain PG2. PCR amplicons were sequenced and results were comparatively scrutinized against those already registered with the M.agalactiae MLST databases. The diversity of repeated sequences in M.agalactiae was conducted using polymerase chain reaction.Results:In order to investigate the genomic relations between these strains, MLVA and MLST strategies concentrating on Target genes was employed. Results analysis by both methods showed identical genetic pattern between all the three vaccine strains. The result of this study indicated that three vaccine strains belonged to the closed clonal complex.Conclusion:If using these strains as vaccine for all the past consecutive decades has had major impacts in population and epidemiology of M.agalactiae in this country, much more work is required. Extending this study to include larger number of local isolates will help to better assess this observation

کلیدواژه ها:

MLST ، Agalactia ، MLVA