COMPARISON THE EFFECTS OF DANTROLENE AND 2-AMINOETHYL DIPHENYLBORINATE ON XBP-1 MRNA GENE SPLICING IN C57 MICE TREATED WITH PSEUDOMONAS AEROGINOSA ENDOTOXIN ISOLATED FROM BURNED PATIENT S LESION

Background and Aim:Pseudomonas aeruginosa is an opportunistic pathogen isolated from burn wounds which is the fourth cause of trauma and death in the world beside HIV and other fatal infectious disease. Research related to mortality in burn patients indicate that Pseudomonas aeruginosa endotoxin (LPS) might be start apoptotic cell death as a result of excessive calcium ions overload through the calcium channels (Like L type channels and mitochondrial IP3 channels). That’s why we select to different specific calcium channels blockers (dantrolene and 2-Aminoethyl diphenylborinate) to investigate their probable supportive role due to vital unfolded protein response (UPR) activation beside LPS inducing endotoxicity in burned patients.Methods:lipopolysacharide was extracted using specific extraction kit. Intraperitoneally (IP) injected C57BL/6 male mice were ethically sacrificed and their spleen removed after 2, 8 and 24 hours after treatment.Considering aseptic-RNase free condition, Extracted RNA from removed spleen was applied for. cDNA synthesis After Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), the unfolded protein response pathway through IRE-1 stress sensor activation was evaluated via agarose gel electrophoretic pattern.Results:Evidence shows co-administration of dantrolene and 2-Aminoethyl diphenylborinate along with LPS, may be a potent activator of IRE-1 route activation of UPR (XBP-) and calcium channel blockers, seems to be a useful supportive treatment in burned patients.Conclusion:This is a pilot study on susceptible animal model and more studies seems to be necessary for final order of burned lesions treatment

کلیدواژه ها:

burned lesions ، Pseudomonas aeroginosa ، endotoxin ، calcium channel blockers ، unfolded protein response ، XBP-1