Purpose the Present Study Was Done to Determine the Ameliorative Effect Of Polyunsaturated Fatty Acid Omega-3 Against Experimental Diabetes-Induced Damages on Testicular Tissue, Sperm Parameters and Preimplantation Embryo Development in Rat Model

Methods Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg−1 streptozotocin, ip) was induced in test group andsubdivided into non-treated diabetic, 300 and 600 mg kg−1 omega-3-treated (orally by gavage) groups. The rats in control group received 0.5 ml saline using intra gastric gavage. Following 45 days, general histopathological changes, serum level of testosterone, inhibin B, glucose, and sperm parameters, testicular total antioxidant capacity (TAC) and malondialdehyde (MDA) content were analyzed. The mitochondriadependent apoptosis was investigated by assessing the Bcl-2 and caspase-3 expression as well asDNA fragmentation. Finally, the in vitro fertilization (IVF) potential was examined by evaluating preimplantation embryo developing.Results: The omega-3 signifcantlyameliorated the diabetes-induced histological damages, diminished serum level of glucose, testicular MDA content, and enhanced the serum testosterone, inhibin B and testicular TAC. The animals in omega-3-treated groups exhibited a significant (p < 0.05) up-regulation in Bcl-2, as well as remarkable (p < 0.05) down-regulation incaspase-3 expression compared to non-treated diabetic rats.Conclusion Our data showed that the omega-3 (especially at 600 mg kg−1dose level) effectively ameliorates the experimental diabetesinduced infertility in rats by up regulating the testicular endocrine and antioxidant statuses, preventing mitochondria-dependent apoptosispathway and potentially improving the sperm quality.

کلیدواژه ها:

Diabetes • Omega-3 ، Apoptosis ، Oxidative stress ، In vitro fertilization