Effects Of Vitamin D an Activity And Expression Of Superoxide Dismutase and Glutathione Peroxidase in Granulosa Cells of PCOS Patients Compared To Healthy Women

Background: Polycystic ovary syndrome (PCOS) is affected millions of women worldwide and accompanied by oxidative stress (OS) which could be because of antioxidant deficiency. We assessed activities and expression of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in granulosa cells (GCs) of PCOS and healthy women. Regarding to positive influences of vitamin D on female reproduction and gene expression, we tested vitamin D effects on these antioxidant enzymes. Methods: GCs were obtained from 20 women with PCOS and 20 healthy controls. Ovarian GCs were cultured in presence or absence of vitamin D (100 nM), for 48 hours. The GPx and SOD expression and activities in GCs lysate were assessed by quantitative real-time PCR and photometric methods. The comparison between two groups was done by independent t-test and the effect of vitamin D tested by paired t-test. All statistical analysis performed using GraphPad Prism. Results: Basal expression and activity of GPx in cystic GCs was lower than normal cells (P<0.0001). Treatment with vitamin D significantly increased GPX and SOD expression and activities in both groups. Vitamin D was more effective on GPx gene expression and activity of normal cells than cystic cells. onclusion: We showed GPx deficiency in patients GCs that could be partly explaining OS in PCOS. The current research is the first one demonstrated the antioxidant properties of vitamin D by enhancing the expression and activity of SOD and GPx in human GCs. In this way, vitamin D supplementation could be useful to attenuate OS in women with PCOS.

کلیدواژه ها:

Polycystic Ovary Syndrome ، Granulosa Cells ، Vitamin D ، Superoxide Dismutase ، Glutathione Peroxidase