Investigation of Genetic Diagnostic Components of Sperm Stem Cells (Ssc) in Moghani Sheep

The proliferation and enrichment of spermatogonial stem cells in the culture medium is important because the colonization of these cells is a valuable source of germ cells for subsequent studies such as freezing , the transplantation of these cells in the treatment of infertility, genetic manipulation and differentiation in the laboratory environment and maintain fertility in cancer patients. In order to extract the reliable source of stem cells, testicular tissue of Moghani sheep was examined as a sample and during the four stages, the collection and preparation of testicular tissue, tissue enzyme digestion, cell transfusion from cells and spermatogonial stem cells were performed. In the case of six genes that are expressed as spermatogonia (c-KIT2 - THY1, PLZF, PGP9.5, DAZL, VASA) properly identifying spermatogonia gene specific primers weredesigned and constructed. After cell cytoplasmic RNA extraction, RT-PCR was obtained from the mRNA that were designed using primers, cDNA synthesis was perfofmed and its amount was amplified by 35 cycles and electrophoresis was performed. In the study of bands obtained from electrophoresis under ultraviolet light, it was determind that the genes were expressed in spermatogonia. The results showed that cell cultyre from isolation and proliferation of these cells is suitable for testicular tissue of eagle sheepwas appropriate and cells that grow beyond the ability to express pluripotent periods in acceptable and non-differentiated media.

کلیدواژه ها:

Azoospermia ، Spermatogonian ، Moghani Sheep ، Adult Stem Cells ، RT-PCR