Spectroscopic Studies on the Interaction Between Etofylline and Human Serum Albumin

Human serum albumin (HSA) principally tasks as a transport carrier for a vast variety of naturalcompounds and pharmaceutical drugs. The present work is devoted to investigate the molecular interaction of 1,3-Dimethyl-7-(hydroxyethyl) xanthine (etofylline) with HSA under physiological conditions, using UV-visible andfluorescence spectroscopies. Fluorescence quenching titration carried out in 50 mM sodium phosphate buffer, pH 7.4 atdifferent temperatures (298, 303 and 310K). Spectroscopic observations along with the Stern-Volmer equation wereemployed to determine the quenching mechanism. Fluorescence data revealed that etofylline quenches the intrinsicfluorescence of HSA through static quenching. The results obtained by spectroscopic studies will be of biologicalsignificance in pharmacology and clinical medicine.

کلیدواژه ها:

Human serum albumin ، Etofylline ، Fluorescence spectroscopy