Study of miR-30-3p expression profile in human breast cancer
محل انتشار: سیزدهمین کنگره بین المللی سرطان پستان
سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 360
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
این مقاله در بخشهای موضوعی زیر دسته بندی شده است:
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICBCMED13_006
تاریخ نمایه سازی: 2 تیر 1397
چکیده مقاله:
Introduction & Aim: MicroRNAs (miRNAs) are small non-coding RNAs that contribute to modulating signaling pathways after radiation exposure and have emerged as a potential therapeutic target or biomarker in the radiation response of cancer. miR-30-3p has tumor suppressive function through the mechanism underlying transcriptional repression of KRT7. This study was aimed to compare miR-30-3p expression profile in human breast cancer using RT-qPCR.Methods: Twenty samples from patients with breast cancer and twenty samples from non-tumor were collected by a pathologist specialist. After RNA extraction from tumoral and normal tissue samples, cDNA was synthesized according to protocol and RT-qPCR carried out using SYBR®Premix Ex TaqTM II kit. miR-30-3p expression profile was analyzed by ΔΔCT method.Results: Our results revealed that the mean of miR-30-3p expression profile in human breast cancer is down-regulated in comparison with control group.Conclusion: Overall, the results of the present study revealed distinct differences in the expression profile of miR-30-3p to control group (down-regulation of miR-30-3p), and this miRNA can be used as biomarkers for cancer therapy.
کلیدواژه ها:
نویسندگان
Roya Gholami
Faculty of Nursing and Midwifery, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
Asghar Arshi
Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran