PI3K gene expression in triple negative breast cancer clinical samples
- سال انتشار: 1394
- محل انتشار: دوازدهمین کنگره بین المللی سرطان پستان
- کد COI اختصاصی: ICBCMED12_200
- زبان مقاله: انگلیسی
- تعداد مشاهده: 544
نویسندگان
MSc Student of Clinical Biochemistry, Student Scientific Research Center (SSRC), Semnan University of Medical Sciences (SEMUMS), Semnan. Iran
MSc Student of Clinical Biochemistry, Student Scientific Research Center (SSRC), Tehran University of Medical Sciences (TUMS), Tehran. Iran.
Ph.D. of Medical Biochemistry, Associated Professor, Department of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan,, Iran
Ph.D. of Medical Biotechnology, Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Introduction & Aim: Triple negative breast cancer (TNBC) is very invasive and a lethal form of breast cancer. In molecular point of view, deregulation of signaling pathways can lead to many disorders, like breast cancer. Among this, PI3K pathway is a main pathway in cell growth and development. Deregulation of PI3K signaling genes is reported in a variety of disease and cancers. PI3K is the key gene in this pathway,so the aim of this study was to assess the expression of PI3K gene in TNBC samples. Methods: 20 tumoral and 20 normal adjacent breast samples were received from Tumor Bank, Cancer Institute, Imam Khomeini Hospital (Tehran, Iran). RNA extraction, cDNA synthesis and Quantitative Real- Time RT-PCR analysis were performed. REST 2009® software was used for final relative expression analysis. The result of Gene expression was normalized to HPRT1 as a house keeping gene. P-value less than 0.05 was considered as significant level Results: Data Analyses showed that PI3K expression was significantly increased up to 4 folds in tumor samples compared with normal samples (P< 0.05). Conclusion: PI3K pathway plays an important role in cell proliferation and migration, particularly ininvasive TNBC. Overexpression of PI3K gene can lead to differentiation, progression and invasion of breast tumor. More studies are needed to find out the real role of this gene in TNBC. The results of this study can have a contribution in diagnosis, prognosis and treatment of breast cancerکلیدواژه ها
TNBC, PI3K, quantitative real-time PCRاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.