Effect of Curcumin on Fatty Acid Synthase expression and enzyme activity in breast cancer cell line SKBR3
- سال انتشار: 1394
- محل انتشار: دوازدهمین کنگره بین المللی سرطان پستان
- کد COI اختصاصی: ICBCMED12_141
- زبان مقاله: انگلیسی
- تعداد مشاهده: 472
نویسندگان
۱Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
۱Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran.
Department of Medical Genetics, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran,Iran
Department of Laboratory Medicine, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran
چکیده
Introduction: Fatty acid synthase is a multifunction protein that catalyzes de novo synthesis of longchain fatty acids. FASN expression is higher in HER2-positive cells, such as SKBR3 and MCF-7/HER2 cells, than in MCF-7 cells, which express lower HER2 levels. Curcumin, a yellow-colored hydrophobic polyphenol derived from the rhizome turmeric, significantly suppressed growth of human breast cancer cells. In this study, we assessed the effect of curcumin on expression and activity of fatty acid synthase in SK-BR-3 breast cancer cells.Aim: In this study, we decided to determine effect of Curcumin on Fatty Acid Synthase expression and enzyme activity in breast cancer cell line SKBR3.Materials and Methods: We assessed the cytotoxicity effect of curcumin in SK-BR-3 cells by MTT. Apoptosis was performed by flow cytometry. FAS activity was measured by a spectrophotometer at 340 nm of NADPH absorption. Fatty acid synthase gene expression was analyzed by real-time PCR Results: Curcumin could decrease cell viability and induce apoptosis in SK-BR-3 cells. Curcumin also reduces the enzyme activity and expression of fatty acid synthase. Conclusions: It is possible that inhibitory effect of curcumin on FAS may induce apoptosis in SK-BR-3 breast cancer cellsکلیدواژه ها
fatty acid synthase, curcumin, breast cancer cells, apoptosisمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.