In Vitro Anti- Candida Activity of Silver Nanoparticles and Fluconazole on Candida glabrata

Candidiasis is one of the most important opportunistic fungal diseases which Candida albicans was considered as the most important factor of it for a long time. But today, with the prevalence of non-albicans, C. glabrata, in particular, it s prevalence is lower. The most common antifungals for the treatment of Candida infections are azoles. But today, because of resistance of Candida species to azoles, especially fluconazole, we need to look for new therapies. Studies have shown that silver nanoparticles have antimicrobial effects against some fungi and bacteria. The purpose of this study is examine the antifungal activity of silver nanoparticles and fluconazole on Candida glabrata.Methods: Antifungal activity of silver nanoparticles and fluconazole against the fungus Candida glabrata, was examined in agar dilution method. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the fungus were determined in the microdilution method in the culture of sabro dextrose broth.Results: In agar dilution method, the concentrations higher than 15/62 ppm of silver nanoparticles and 250 μg/ml of fluconazole, Candida glabrata does not grow. MIC of silver nanoparticles was 15/62 ppm and fluconazole was 250 μg/ml and MFC of silver nanoparticles was 31/25 ppm and fluconazole was 500 μg/ml.Conclusion: According to the results, silver nanoparticles had the lowest rate of growth of fungus colonies. In general, results of this study showed that silver nanoparticles can be useful as an anti-candida and further studies may be an adequate replacement for the azoles

کلیدواژه ها:

Candida glabrata ، silver nanoparticles ، fluconazole