Association of Endothelin-1 rs5370 G> T gene polymorphism with the risk of nephrotic syndrome in children

Background: Primary nephrotic syndrome (NS) is a common kidney disease in children.Objectives: The present study was aimed to investigate whether rs5370 G> T (lys198Asn) genetic variant of endothelin-1 (ET-1) is involved in the susceptibility to NS.Patients and Methods: This case-control study was performed on 138 patients with NS and 150 healthy children. Genomic DNA was extracted from whole blood using salting out method. Polymorphism of the ET-1 rs5370 G> T (lys198Asn) polymorphism detected by T-ARMS-PCR as well as PCR-RFLP method.Results: The results showed that the genotype and allelic frequencies of the ET-1 rs5370 G> T variant were not significantly different between cases and controls. Furthermore, subgroup analysis showed that rs5370 G> T variant was not associated with gender of patients. In NS patients the genotype was not associated with cholesterol, triglyceride, total protein and albumin levels.Conclusions: In conclusion, our findings indicate that ET-1 rs5370 G> T is not associated with NS.Further studies with larger sample sizes and different ethnicities are required to validate our findings