Protective effect of omega-3 on the quality of sperm and in vitro fertilization in diabetic adult male rats

Background: Diabetes is one of the determinants of the fertility that can disrupt ovulation and formation of the fetus. The aim of the present study was to determine the ameliorative effects of the polyunsaturated fatty acid Omega-3 on sperm parameters, in vitro fertilization (IVF) and embryo development in diabetic rats.Methods: 32 mature male rats were divided into four groups as the control, diabetic (50mg/kg sterptozotocin), intraperitonealy (IP), Diabetic+Omega-3(Low-Dose 300mg/kg b.w-1) and diabetic+Omega-3(High-Dose 600mg/kg b.w-1). Following 45 days, the rats were euthanized and epididymis tail was transferred to the human tubular fluid (HTF) medium containing 4 mg mL-1 bovine serum albumin (BSA). The sperm count, percentage of live, immature sperms and chromatin condensation were investigated. Moreover, it is necessary to mention that the in-vitro-fertilization potential of sperms was analyzed. The oocytes were obtained from immature rats after the injection of pregnant mare s serum (PMSG) and human chorionic gonadotropin (HCG) hormones. Human tubular fluid was used as the fertilization medium and zygotes transferred to fresh 1-cell rat embryos culture medium (mR1ECM) to reach the blastocyst stage.Result: The omega-3 significantly (P Conclusion: The results obtained from this study indicated that the oral administration of omega-3 increases the fertility in rats by influencing the sperm parameters.

کلیدواژه ها:

Fertility ، Omega-3 ، Rat ، Sperm ، Diabetes