Serum of spayed female dog and superovulation response in rat and mice

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 413

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

ISERB03_390

تاریخ نمایه سازی: 11 خرداد 1397

چکیده مقاله:

Background: The researchers are trying to find and improve production process of superovulation hormones. In spayed female dogs due to loss of negative feedback of estrogen and progesterone, FSH concentration increase in serum. It may induce superovulation by stimulation of follicular development.Methods: Rats at diestrus stage aligned in PMSG group (30 IU PMSG, 48 hours later, 25 IU of hCG), rFSH group (for 48 hours, once every 12 hours by reducing dose of 5, 4, 3, 2 and 1 unit rFSH, and 25 units of hCG with last injection) and group of dog’s serum (for 48 hours, once every 12 hours by reducing dose of 0.6, 0.4, 0.3, 0.2 and 0.1 ml, and 25 units of hCG with last injection). Three groups of mice were PMSG (at 13pm, 5 units of PMSG, 48h later 5 units of hCG), rFSH (from 13pm, for 48 hours once every 12 hours and reduce dose of 2.5, 2, 1.5 , 1 and 0.5 units of rFSH, 5 IU hCG with the last injection) and serum of anestrous dog (from 13pm, for 48 hours every 12 hours and reduce dose of 0.1, 0.075, 0.05, 0.03 and 0.025 mL, 5 IU hCG with the last injections). Immediately after hCG injection, females were placed at a ratio of 1 to 1 with males for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and ovarian samples fixed in buffered formalin 10% for histopathological evaluation and corpus luteum counting. Data analyzed with Graphpad Prism software version 6 and One way ANOVA and Turkey’s test. The significant level considered PResult: There was no significant difference among rats groups (P=0.066). Also, mean differences between rat groups were not significant (P> 0.05). The number of CLs was significantly differed among mice groups (P=0.0057). The mean number of CLs between FSH and dog serum (P=0.01) and PMSG and FSH (P=0.0182) showed significant differences.Conclusion: Serum of spayed female dog could not induce superovulation in rats but it could induce superovulation response in compare with PMSG in mice.

کلیدواژه ها:

نویسندگان

Mohammad Hossein Noorazni zadeh

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Asghar Mogheiseh

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Masood Sepehrimanesh

Gastroenterohepatology Research center, Shiraz University of Medical Sciences, Shiraz, Iran

Mojtaba Kafi

Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran