Protective effect of royal jelly and vitamin C on sperm parameters and in vitro fertilization in mice with hemolytic anemia

Background: Hemolytic anemia induced by Phenyl hydrazine (PHZ) can cause disorders in fertility and spermatogenesis. This study aims to assess the effects of vitamin C and royal jelly as a protective antioxidant compounds against the PHZ-induced hemolytic anemia.Methods: 32 adult male mices were divided randomly into four groups (n=8). The first group received 0.1 ml/day of saline intraperitoneally (IP).The second group received 60 mg/kg/48-hours of phenyl hydrazine IP. The third group received along with phenyl hydrazine, 250 mgkgday of vitamin C IP and 100 mgkgday of royal jelly by gavage. The fourth group received the same doses of vitamin C and royal jelly as the third group. After 35 days, serum samples were obtained, the sperms were collected from epididymis and in-vitro fertilization (IVF) was evaluated.Result: The results of this study showed that the hemolytic anemia induced by PHZ significantly decreased serum testosterone concentration and at the same time increased the amount of nuclear DNA damage and immature nucleuses sperms. Also decreased the number of fertilized oocytes, two-cell and four-cell embryos, morula, blastocyst and arrested embryos. However, administrating of royal jelly and vitamin C improved these parameters significantly (p Conclusion: It seems that royal jelly and vitamin C as free radical scavengers have the potential to decrease oxidative damages on reproductive organ in hemolytic anemia induced by PHZ.

کلیدواژه ها:

In vitro fertilization ، Phenyl hydrazine ، Royal jelly ، Sperm parameters ، Vitamin C ، Hemolytic anemia