The protective effect of Royal jelly on in vitro fertilization capacity of sperm in adult male rat alteration following heat stress

Background: Heat stress, including an increase in paternal temperature, causes a decline fertility in human and mammalian. To achieve male subfertility, testes of adult male mice were immersed in water at either heated (42-39-37°C) during 20 min. The present study aimed to evaluate the protective effect of Royal jelly (RJ) on fertility rate alliteration following heat stress in male rats.Methods: This experimental study was conducted on 40 adult male Wistar rats.The animals were divided into 8 groups (n=5 per group): control, RJ, 43℃, 39℃ 37℃, 43℃ in water bath for 20 mins). RJ, at a dose of 100 mg/kg BW was given by gavage. The duration of treatment was 48 days. At the end of 48 days fasted rats were killed. After removal of testis the tail of epididym was isolated and transferred to HTF medium. Epididymal sperm fertilizing capacity of all animals evaluated following in vitro fertilization, data were analyzed using one way ANOVA and tukey test.Result: The results showed that fertilization rate decrease significantly (p < 0.05) in heat stress alteration groups in compared to control and RJ groups. Overall, RJ administration to heat stress groups ameliorate fertilization rate. There no significant difference (pConclusion: These results indicate that oral administration of royal jelly could improved adverse effect of alteration heat stress on fertilization rate and oocyte fertilized.

کلیدواژه ها:

Fertility ، In vitro fertilization ، Rat ، Royal jelly ، Heat stress