Effect of estrus cow serum (ECS) and human menopausal serum (hMS) on bovine oocyte in vitro maturation

Background: In vitro maturation of oocyte (IVM) is one of the most important subjects that has been studied in reproductive biotechnology. The aim of this study was to compare the effect of adding estrus cow serum (ECS) and human menopausal serum (hMS) with different concentrations on IVM of bovine oocyte in order to improve IVM rate of oocytes.Methods: Oocytes collected from slaughterhouse bovine ovaries and treated in media with different types of serum. Maturation used in this study was divided into five categories: DMEM (control), DMEM + ECS10% (group 1), DMEM + ECS20% (group 2), DMEM +hMS10% (group 3), DMEM + hMS20% (group 4). Significant differences between treatments were evaluated by GLM test with SAS 9.2 program followed by the Chi-square and Fisher s exact test.Result: Based on the results, supplementation media with hMS20% and hMS10% caused the increase of IVM percentage (47/82%, 33/33%) respectively. IVM ratio between group 1 and group 4 was significantly different (P0/05).Conclusion: It can be concluded that using high concentration of hMS and ECS in comparison with control group cause no significant difference on in vitro maturation of bovine oocytes. Also, for the reason of preventive effects of estradiol that exist in ECS, adding this serum into oocyte maturation media is not recommended.

کلیدواژه ها:

Bovine ، Human menopusal serum ، In vitro maturation ، Oocyte ، Estrus cow serum