The effect of platelet reach fibrin encapsulation on reducing apoptosis and oxidative damage in mice vitrified ovaries following autotransplantation

Background: Cancer therapeutic agents are cytotoxics for germ cells, thus infertility is common among young cancer survivors. Ovarian transplantation is known to restore fertility but its major obstacles appear to be ischemia/reperfusion (IR) injury which leads to apoptosis. The aim of this study was to investigate the effects of ovarian tissue encapsulation in platelet reach fibrin (PRF) on apoptosis incidence and oxidative damage following ovarian autotransplantation in the gluteus superficial muscle.Methods: NMRI mice (4-5 weeks age) were divided into three groups (n=6): control (freshly isolated ovaries), vitrified autografted(the ovarian tissue were encapsulated in PRF just before transplantation) and vitrified autografted +PRF. 7 days after ovarian transplantation, the plasma concentrations of MDA and FRAP were measured and 28 days post transplantation, the apoptotic rate of follicles was also estimated. Data were analyzed using one-way ANOVA and Tukey s test and the means were considered significantly different at pResult: The level of MDA significantly increased and the level of FRAP significantly decreased in the vitrified autografted group compared to the control. While the mentioned parameters were ameliorated to the control level in the vitrified autografted + PRF group. Apoptosis rate in the vitrified autografted+PRF group reduced significantly relative to the vitrified autografted group.Conclusion: Ovarian tissue encapsulation in PRF reduces the oxidative stress and apoptosis; therefore it can be suggested as a new effective approach to ovary transplantation with fewer side effects.

کلیدواژه ها:

Ovary ، Oxidative stress ، Platelet reach fibrin ، Vitrification ، Transplantation