The effect of ICV injections of calcitonin gene-related peptide and salmon calcitonin on sperm fertility of rats

Background: Hypothalamic-pituitary-gonadal axis plays an important role in the regulation of fertility. The aim of this study was to evaluate the effect of ICV injection of calcitonin gene related peptide (CGRP) and salmon calcitonin (sCT) on fertility parameters of rat sperm.Methods: Eighteen male rats were divided into three groups (n=6). In the first group normal saline was injected into the ventricle (5 μl). CGRP in the second group and sCT in the third group were injected into the brain ventricle (1.5 nmol). After surgery epididymal sperms were used to assess mobility, density, and survival. The data were analyzed by using SPSS software with statistical method of the one-way ANOVA.Result: In this study, CGRP significantly reduced sperm motility and density compared to the control group. sCT only reduced sperm density compared to the control group. CGRP reduced live spermatozoa and intact acrozome compared to the control group and increased dead spermatozo, loose acrosome, and damaged acrosome. sCT decreased intact acrozome and increased loose acrosome and damaged acrosome compared to the control group.Conclusion: After ICV injection of CGRP and sCT, sperm fertility in the rats was reduced. In this regard, it was observed that the effect of CGRP in some parameters was higher than sCT. These effects may be due to a reduction in FSH and LH pulses.

کلیدواژه ها:

Fertility ، sCT ، Sperm ، CGRP