The effect of troxerutin administration during prepubertal period on total number of spermatogenic cells in type I diabetic rats

Background: It is believed that the production of reactive oxygen species and development of oxidative stress is one of the major events for testis damage in diabetic patients. Troxerutin (TX) is a trihydroxyethylated derivative of the natural bioflavonoid rutins and commonly known as vitamin P4, possess well-proven antioxidant activities but there is no report to investigate whether troxerutin has a supportive effect on spermatogenesis process in diabetic peoples.Methods: Fifty prepubertal (2 weeks old) male wistar rats were divided into five groups including: Control, Diabetic (DM), TX, DM+Insulin and DM+TX. Type I diabetes was induced by a single dose of streptozotocin (IP, 55 mg/kg). Treatment groups received troxerutin (OG, 150 mg/kg/day) and insulin (SC, 4-6 IU/day) for 4 consecutive weeks. Finally, animals euthanized and then left testes were fixed in buffered formalin 10%. The samples were processed by standard paraffin embedding and serially sectioned at 20 μm thickness. Twenty to twenty-five sections per animal were selected through systematic random sampling and stained by H&E. Total numbers of spermatogenic cells were estimated by optical dissector and stereo-investigator system using an unbiased counting frame.Result: Stereological studies showed that diabetes decreased total number of all spermatogenic cells significantly (pConclusion: Based on our results, it can be concluded that administration of TX is a suitable protective strategy for side effect of diabetes in testis of prepubertal diabetic males.

کلیدواژه ها:

Diabetes ، P4 ، Prepubertal ، Spermatogenic cells ، Troxerutin