The effect of royal jelly on sperm concentration and motility in immobilization stress-exposed male mice

Background: In this modern world, stress is an unavoidable phenomenon. Immobilization as a model of stress produces an inescapable physical and mental stress. Several reports have suggested a stress related decline in semen quality, sperm concentration, morphology and percentage of motility. Royal jelly is mainly made of proteins, sugars, lipids and small amounts of mineral salts and vitamins. The amino acid content of royal jelly may play a role as well by enhancing acrosome reaction, sperm motility, or improving fertilization.Methods: In this study, 40 male mice were divided into five groups containing eight animals in each groupincluding:1-control group 2-immobilization stress group 3,4 and5 Immobilization stress + orally royal jelly group at doses of 50,100 and 200 mg /kg body weight of mice. At the end of treatment animals, were sacrificed. The epididymal sperm count was determined by hemocytometry (Neubauer chamber) using the method described in the WHO manual. In order to observe mobility, 10 microliters of semen was placed on a glass slide and using a light microscope with a magnification of ×40, were counted in several microscopic fields.Result: According to this study, the count and motility of sperm in the stress group compared to the control group showed a significant decrease (pConclusion: Royal jelly improved adverse effects of chronic immobilization stress on sperm concentration and motility.

کلیدواژه ها:

Fertility ، Male mice ، Royal jelly ، Immobilization stress