Sperm content of PLC-ζ protein is related to semen analysis parameters

Background: PLC-ζ deficiency in sperm can underlie oocyte activation failure after ICSI. Fertilization failure during in vitro fertilization (IVF) occurs in approximately 4%–7% of couples. It is believed that PLC-ζ expression varies notably in sperm from normal fertile males, and it may vary between ejaculates from the same person. In This study sperm content of PLC-ζ protein was evaluated by flow cytometry correlated with clinical semen analysis parameters.Methods: Fresh semen samples were obtained from 30 men who attended the Andrology Unit of the Shahid beheshti hospital Fertility and Infertility Center. After semen liquefaction semen analysis performed according to the World Health Organization (WHO, 2010) criteria. Sperm samples were processed by density gradient. Flow cytometry was used as a quantitative method for the measurement of sperm PLC-ζ content. Pearson correlation coefficient was calculated to measure correlation between the distribution patterns of PLCζ and the different sperm parameters.Result: The mean sperm concentration was determined in the semen analysis was 69.56 ± 11.4 million/ml (mean±SD) with ranged from 8 to 233 million/ml means sperm mo tility was 40.08 % ± 11.3 as well as mean sperm normal morphology was 7.6 ± 0.39 with a mini mum and maximum 5 to 12%.There was positive correlation between the levels of PLC-ζ and normal morphology (r=0.6 P=0.002). However, no significant correlation was observed between PLC-ζ expression, motility or concentration.Conclusion: Our clinical data demonstrate significant correlations between sperm PLC-ζ levels and normal morphology in sperm

کلیدواژه ها:

Intracytoplasmic sperm injection ، Sperm ، Flow cytometry ، Oocyte activation ، Phophospholipase C zeta