Evaluating genetic alterations of the first exon and intron of TEKT2 gene in infertile men with total sperm immotility referring to Royan institute

Background: Sperm immotility is the absolute asthenozoospermia that sperms have no motility at all. It may be due to several reasons including ultrastructural defects like dysplasia of the fibrous sheath (DFS) or primary ciliary dyskinesia (PCD), and mutation in genes which involve in sperm flagella formation. The aim of this study was to evaluate genetic alterations in first exon and intron of TEKT2, a gene which has important role in formation of inner dynein arm of sperm flagella and may have a role in spermatogenesis.Methods: In this case-control study, blood samples were retrieved from 20 infertile men with total sperm immotility as case group and 20 men with normal spermogram as control group. DNA was extracted from these blood samples, then the mentioned region amplified in both case and control groups by PCR using specific primers. Finally, these amplified regions were sequenced in order to evaluate any alteration.Result: After PCR-sequencing, no variations have been observed in both case and control groups, compared with reference sequence.Conclusion: From obtained results, it could be concluded that genetic alterations of first exon and intron regions of TEKT2 have no relation to sperm immotility. The evaluation of other exons/introns of this gene in order to find alterations related to sperm total immotilty is strongly recommended.

کلیدواژه ها:

Flagella ، Infertility ، TEKT2 ، Sperm immotility