Study of interaction of hemoglobin protein with malathion using circular dichroism and fluorescence spectroscopy

department biochemistery biophisic, university eslamic azad mashhad, iranIn the peresent work studied interaction between hemoglobin protein and malathion in peresence and absence silver nano particlesewith different three measure by fluorescence spectroscopy. Hemoglobin is important protein in blood beedise that studied binding ligand – protein for the first time. Malathion is kind of toxicant that widly use in agricultur. Malathion is an organophosphorous insecticide with appearance green oil. Silver nan particles widly use in different branches biological science. In this work studied effect nanoparticles in scale assembling malathion per hemoglobin. The results of uv/vis fluorescence spectera showed distance ligand and protein is 2.75 nm. Also in presence silver nanoparticles 1,2&3 this distanse is 2.63, 2.72&2.68 nm. Studied effect malathion over secondery structure hemoglobin protein with circular dicroism flourescence. The results showed changes thus level α – helix hemoglobin changed from 45.84 to 41.71.

کلیدواژه ها:

hemoglobin ، malathion ، silver nanoparticles ، circular dicroism flourescence ، uv/vis