Evaluating the effects of Cucurbitacin D on the mortality of Breast Cancer cell line MDA-MB 468 using Colorimetric MTT assay

سال انتشار: 1394
محل انتشار: یازدهمین کنگره بین المللی سرطان پستان
کد COI اختصاصی: ICBCMED11_062
زبان مقاله: انگلیسی
تعداد مشاهده: 420

نویسندگان

Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

چکیده

Introduction: Breast cancer is the second most common cancer and the leading cause of cancer death in women. Cucurbitacins are highly oxidized tetracyclic triterpenoids found in medicinal plants known for their diverse pharmacological and biological activities, including anticancer effects. The aim of the present study is to examine the effects of the anti-tumor cucurbitacin D on viability of breast cancer cell line MDA-MB 468. Method: The effect of cucurbitacin D on Cell viability was investigated applying colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay. Cells were seeded in a 96-well plate (10,000 cells/well) in culture medium (DMEM/F12) and incubated for 48 h. Then cells were treated with different concentrations of cucurbitacins D (1,10,20-70 μM) for 24 h. Untreated cells were considered as a negative control. One hundred microliter MTT solution (1:5, diluted by medium) was added to each well. After 4 h incubation at 37 ◦C the medium was removed and the remaining formazan crystals were dissolved in DMSO, and the optical density was measured at 570 nm using an ELISA plate reader. Result and Conclusion: The experiment demonstrated that LC50 of cucurbitacin D in MDA-MB468 is 25 μM. It means 50% cell population were killed in this concentration. This result shows high potency of cucurbitacin D.

کلیدواژه ها

Cucurbitacin D, Breast Cancer, MDA-MB468 cell line, MTT Assay

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