Comparison of IL-25 and IL-17B in Apoptosis and Proliferation induction of breast cancer celllines
- سال انتشار: 1393
- محل انتشار: دهمین کنگره بین المللی سرطان پستان
- کد COI اختصاصی: ICBCMED10_062
- زبان مقاله: انگلیسی
- تعداد مشاهده: 512
نویسندگان
Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
چکیده
Cytokines are one of the major components of tumor microenvironment. IL-25(IL-17E) and IL-17B are two members of IL-17 family which both of them bind to a unique receptor (IL-17RB). Sincethereceptoris widelyexpressedinbreast cancertissue, so the role of these cytokines in the tumor fate can be important.Materials and Method: MCF-7 (Estrogen positive) and MDA-MB231 (Estrogen negative) as breast cancer cell lines were treated by human recombinant cytokines (rhIL-25/IL-17E) and rhIL-17B from R&D system). Apoptosis test was performed using Annexin V and PI staining and analyzed by flow cytometry. Proliferation rate was evaluated by MTT proliferation assay. Results: According to our results apoptosis rate in IL-25 treated cells was more than control cells and proliferation rate in IL-17B treated cells was increased. To evaluate cytokines competition, different ratios of cytokines were used. The results showed that in some ratios these cytokines had inhibitory and/or a synergistic effect on each other.Conclusion: Biochemical component of the tumor microenvironment in different circumstances change and cytokines play an important role in tumor cell behavior. It seems these cytokines (IL 17E and IL- 17B) competed with each other and the balance is very important on the fate of breast cancer cells.کلیدواژه ها
Breast cancer, IL-17B, IL-25, MCF-7, apoptosisمقالات مرتبط جدید
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