The Effect of CXCR4-siRNA Nanoparticles on serum ALP and LDH levels in a mouse model of colorectal cancer Metastasis to the liver

Previous researches showed that inhibition of CXCR4 and its ligand CXCL12 signaling by siRNA knockdown has been found to reduce metastasis breast cancer. The aim of this study was to investigate the effect of CXCR4 gene silencing on serum ALP and LDH levels in colorectal cancer by using CXCR4 siRNAs/dextran-spermine nanoparticles. The fabricated nanoparticles were used in order to investigate whether down regulation of CXCR4 expression could affect on serum ALP and LDH levels in mouse models of colorectal cancer. In this study colorectal cancer was established in BALB/C mouse by IV injection of mouse colon carcinoma cells CT.26WT. CXCR4 siRNA targeting two sites of the CXCR4 gene was administered following injection of siRNA encapsulated into nanoparticles and naked siRNA. In vivo animal s data demonstrated that CXCR4 silencing by siRNA nanoparticles significantly down regulated CXCR4 expression when compared with naked CXCR4 siRNA. Interestingly, there was correlation between CXCR4 expression and serum ALP and LDH levels. Dextran-spermine was identified as an effective carrier for siRNA and inhibition of CXCR4 expression at the mRNA level by CXCR4 siRNAs nanoparticles.

کلیدواژه ها:

Cationized dextran ، Colorectal Cancer ، Serum ALP Enzyme ، serum HDL Enzyme ، CXCR4 ، siRNA ، Nanoparticles