Genetic polymorphism of CENP-E and susceptibility colorectal cancer in Ahvaz

cancer pathogenesis: chromosomal instability (CIN) or microsatellite instability (MIN) and CpG island methylator phenotype (CIMP) pathways. Mutations in the genes encoding centromere proteins have been identified as a stimulating factor in the formation of aneuploidy and tumorigenesis. Centromere-associated protein-E (CENP-E) is a kinesin-like centromere protein that required for efficient capture of spindle microtubules by kinetochores and for efficient chromosome segregation. Therefore, defect in this protein causes an aneuploidy (CIN) and CIN is a hallmark of most solid tumors. We have analyzed an important polymorphism in CENP-E gene which have been implicated in the development of CRC.MATERIAL AND METHOD: In this case-control study, 100 patients with colorectal cancer and 100 healthy controls participated (from hospitals in Ahvaz). We used from polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis for CENP-E Tyr63His detection. Finally, 70 samples (35 case and 35 healthy control) were sequenced by Sanger method for validation of the RFLP results. Frequency were constructed using the SPSS (24.0 version) and threshold for significance was p< 0.05.RESULT:Cases and controls were matched on age, sex and ethnic characteristics, and no significant difference were between them (P = 0.34, 0.77 and 0.61, respectively). There was no significant difference between patients and healthy controls. Also, there was no significant association between genotypes and susceptibility colorectal cancer. The allele frequency was similar between patients and healthy controls, and there was no significant difference between the two groups.CONCLUSION:This polymorphism was studied in Ahwaz, southwest of Iran, The CENP-E Tyr63His (A/G) polymorphism may be associated with colorectal cancer in other Asians, but it appears to have no such effect in Southwest of Iran. The study also highlights the importance of conducting genetic association studies in different ethnic populations.

کلیدواژه ها:

Colorectal Cancer ، Polymorphism ، CENP-E ، susceptibility ، Ahvaz