The demolition of neural cell cancer by gold nanoparticle coated with lysine
- سال انتشار: 1396
- محل انتشار: سومین سمپوزیوم بین المللی سرطان نسترن
- کد COI اختصاصی: NASTARANCANSER03_263
- زبان مقاله: انگلیسی
- تعداد مشاهده: 495
نویسندگان
Student Research Committee, Department Of Immunology, Faculty Of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran
Department Of Pharmaceutics, School Of Pharmacy, Mashhad University Of Medical Sciences, Mashhad,Iran
Student Research Committee, Department Of Immunology, Faculty Of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran
Student Research Committee, Lorestan University Of Medical Sciences, Khorramabad, Iran
چکیده
At present, cancer is the second leading cause of death in the world, so finding a way to eliminate these cells is required. The aim of this study was to investigate the mechanism of degradation of goldnanoparticle coated with lysine amino acids on PC12 category of cancer cell. In this study, PC12 cell category was cultured in vitro and these cells divided into 7 groups and then 6 groups exposed for 48hours to varying concentrations of gold nanoparticles (0.5, 2.5 and 5 μM gold nanoparticles, Gold nanoparticles coated with lysine) and a group without nanoparticle as the control. The effect of nanoparticle cytotoxicity was examined by using MTT and LDH release from the cells. The phase contrast microscopy was used to evaluate the cellular morphology. Finally, the level of ROS in the cells was measured by using Fluorescent probe of 2, 7 Dichloroisocyanurate fluorescein Diacetate. The average diameter of the gold nanoparticles in the case of uncoated and coated with lysine amino acids, respectively 10 ± 0.2 and 29 ± 0.3 nm was calculated. The most damaging effect of the studied cells by micro-molar concentrations of 25 gold nanoparticles coated with lysine amino acid, which occurred at a rate of 40% compared to the group treated with bare gold nanoparticles was a statistically significant difference (p≤ 0/05). The results of this study indicate that the majority of apoptosis of cells incubated with uncoated and coated gold nanoparticles is as apoptosis. The results showed that gold nanoparticles coated with lysine amino acid increased ROS and apoptosis mechanisms in cancer cells causing cell death in PC12 categoryکلیدواژه ها
Cell and Cancer, Nutrition and Cancer, Multidisciplinary Cancer Research, Cancer Genetics, Targeted Cancer Therapyاطلاعات بیشتر در مورد COI
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