DNA Binding and Biochemical Investigation of Cu (II) Complex Containing 1,10- phenanthroline and Iminolactone

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 488

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:


تاریخ نمایه سازی: 7 اسفند 1396

چکیده مقاله:

Deoxyribonucleic acid (DNA) is the primary target molecule formost anticancer and antiviral therapiesaccording to cell biologists[1]. Over the past decades, there has been a considerable interest inDNAbinding properties towards different types of transition metalcomplexes under physiological conditions[2]. In this work characterization of the interaction between Cu (II) complex containing 1,10-phenanthroline and iminolactone as ligands, with DNA has been carried out by UV absorption,fluorescence spectra and viscosity measurements in order to investigate binding mode. The experimentalresults indicate that the Cu (II) complex binds to DNA and absorption is decreasing in charge transferband with the increase in amount of DNA. The binding constant (Kb) at different temperatures as well asthermodynamic parameters, enthalpy change (ΔH°) and entropy change (ΔS°), were calculated accordingto relevant fluorescent data and Vant’ Hoff equation. The results of interaction mechanism studies,suggested that groove binding plays a major role in the binding of the complex and DNA.K


Jilla Saffari

Department of Chemistry, Islamic Azad University, Zahedan Branch, Zahedan, Iran

Mehri-Sadat Ekrami-Kakhki

Nano Technology Labratory, Esfarayen University of Technology, Esfarayen, Iran