DNA Binding and Biochemical Investigation of Cu (II) Complex Containing 1,10- phenanthroline and Iminolactone

Deoxyribonucleic acid (DNA) is the primary target molecule formost anticancer and antiviral therapiesaccording to cell biologists[1]. Over the past decades, there has been a considerable interest inDNAbinding properties towards different types of transition metalcomplexes under physiological conditions[2]. In this work characterization of the interaction between Cu (II) complex containing 1,10-phenanthroline and iminolactone as ligands, with DNA has been carried out by UV absorption,fluorescence spectra and viscosity measurements in order to investigate binding mode. The experimentalresults indicate that the Cu (II) complex binds to DNA and absorption is decreasing in charge transferband with the increase in amount of DNA. The binding constant (Kb) at different temperatures as well asthermodynamic parameters, enthalpy change (ΔH°) and entropy change (ΔS°), were calculated accordingto relevant fluorescent data and Vant’ Hoff equation. The results of interaction mechanism studies,suggested that groove binding plays a major role in the binding of the complex and DNA.K

کلیدواژه ها:

Cu(II) complex ، DNA binding ، Fluorescence ، Thermodynamic parameters