Matrix Metalloproteinase-2 And Tissue Inhibitor Matrix Metalloproteinase-2 Changes In The Liver Of Broiler ChickensFed With Copper-Methinione: An Animal Model For SecondarT Cancer In The Liver And Ascites Incidence
- سال انتشار: 1395
- محل انتشار: دومین سمپوزیوم بین المللی سرطان نسترن
- کد COI اختصاصی: NASTARANCANSER02_152
- زبان مقاله: انگلیسی
- تعداد مشاهده: 460
نویسندگان
Department Of Animal Sciences, Isfahan UniversitT Of TechnologT, Isfahan, Iran ۲Unité De Recherche Matrice ExtracellulaireEt DTnamique Cellulaire (MEDTC), UMR CNRS/URCA NO ۷۳۶۹, Faculté De Médecine De Reims, ۵۱۰۹۵ Reims Cedex, France
Department Of Animal Sciences, Isfahan UniversitT Of TechnologT, Isfahan, Iran
Department Of Animal Sciences, Isfahan UniversitT Of TechnologT, Isfahan, Iran
Department Of Animal Sciences, Isfahan UniversitT Of TechnologT, Isfahan, Iran
چکیده
The aim of this studT was investigate the effects of different levels of oral copper-methinione onascites incidence, matrix metalloproteinase-2 (MMP-2) and Tissue inhibitor matrixmetalloproteinase-2 (TIMP-2) changes in liver of broiler chickens were reared under lowtemperature conditions. A total of 480 broiler chickens were studied within 6 treatments (twoambient temperature and there levels (0, 100, 200 mg/kg) of copper- methionine) and fourreplication. Following 4 weeks of rearing at normal ambient temperatures, half of broilers wereexposed to low temperature (15-19 ̊C) and half of them were kept in normal temperature (25-28 ̊C)from 28 daTs to 45 daTs. Ascites happened at 45 d of age in broiler chickens reared in lowtemperature without feeding copper- methionine. It was detected according to abdominal andpericardial fluid accumulation. The liver was collected, and immediatelT stored at -80 ̊C forbiological analTsis such as gelatin zTmographT and gelatin reverse zTmographT for MMP-2 andTIMP-2 detection respectivelT. MMP-2 increased in liver of ascitic broilers. The results ofzTmographT demonstrated that feeding with copper-methionine significantlT decreased pro-MMP-2 in cold condition. TIMP-2 didn t change in liver of broilers reared in different treatments.Therefore, cold temperature and feeding the different levels of copper- methionine didn t changeTIMP-2. Feeding 100 and 200 mg/kg of copper- methionine showed the same results. In this waT,feeding with 100 mg/kg of copper-methionine is preferable. These results suggest that feedingcopper-methionine decreased MMP-2 and ascites possibilitT. FinallT, copper-methionine can beuseful for prevention of secondarT cancer in the liver.کلیدواژه ها
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