Effect Of Oral Copper-Methionine On Matrix Metalloproteinase-2 In The Brain Of Broiler Chickens As An Animal Model For Malignant Ascites
- سال انتشار: 1395
- محل انتشار: دومین سمپوزیوم بین المللی سرطان نسترن
- کد COI اختصاصی: NASTARANCANSER02_145
- زبان مقاله: انگلیسی
- تعداد مشاهده: 443
نویسندگان
Department Of Animal Sciences, Isfahan University Of Technology, Isfahan, Iran;Unité De Recherche Matrice Extracellulaire EtDynamique Cellulaire (MEDyC), UMR CNRS/URCA NO ۷۳۶۹, Faculté De Médecine De Reims, ۵۱۰۹۵ Reims Cedex, France
Department Of Animal Sciences, Isfahan University Of Technology, Isfahan, Iran
Department Of Animal Sciences, Isfahan University Of Technology, Isfahan, Iran
Department Of Animal Sciences, Isfahan University Of Technology, Isfahan, Iran
چکیده
This study was aimed to investigate the effect of copper-methinione on matrix metalloproteinase-2(MMP-2) changes in brain of ascitic broiler chickens under cold stress. Ascites was induced bygrowing in cold temperature in broiler chickens from 28 to 45 d of age. 480 one-day-old broilerchickens were randomly assigned to six treatments (two temperature (thermoneutral and coldstress) and there levels (0, 100, 200 mg/kg) of copper- methionine with four replication in eachtreatment. Data were analyzed using the General Linear Model procedure of SAS software.Treatment means were compared by the Least Significant Difference test and P- values less than0.05 were statistically significant. Ascites ( abdominal and pericardial fluid accumulation) weredetected at 45 d of age in broiler chickens reared in cold temperature and fed a diet without coppermethioninesupplementation. The brains were collected, and immediately stored at -80 ̊C forgelatin zymography and gelatin reverse zymography techniques. MMP-2 increased in brain ofascitic broilers. The results of zymography demonstrated that cold temperature increased MMP-2and feeding with copper-methionine was useful in prevention of MMP-2 in cold condition but it wasnot statistically significant. Feeding different levels of copper- methionine in normal temperaturedid not change MMP-2 in brain of broiler chickens. Tissue inhibitor matrix metalloproteinase-2(TIMP-2) was not affected by temperature and different levels of copper- methionine in brain ofbroiler chickens. Therefore, TIMP-2 showed the same results in different treatments. In conclusion,oral copper-methionine can reduce ascites possibility in cold-stressed broiler chickens .کلیدواژه ها
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