Identify New Polymorphisms Of The CDKN2A Gene In Skin Cancer In Mazandaran
- سال انتشار: 1395
- محل انتشار: دومین سمپوزیوم بین المللی سرطان نسترن
- کد COI اختصاصی: NASTARANCANSER02_072
- زبان مقاله: انگلیسی
- تعداد مشاهده: 514
نویسندگان
Department Of Biology, Islamic Azad University, Damghan Branch, Iran
Cellular And Molecular Biology Research Center, Babol University Of Medical Sciences, Babol, Iran
Bu-Ali Sina Hospital Department Of Pathology, University Of Medical Sciences, Sari, Iran
چکیده
In general, there are a large number of genes involved in the development of skin cancer withdifferent molecular pathways including CDKN2A. Given the importance of this type of cancermolecular genetics research can be very useful in determining the involved cancer oncogenes andidentifying new polymorphisms. The aim of this study is identify new polymorphisms of theCDKN2A gene in skin cancer. In this study 100 of paraffin-embedded skin cancer samples and 50control samples from people who referred to the hospital for skin irritation are collected. Beforeextraction, the samples are deparaffinized. Then, the DNA is extracted by tissue DNA extractionmini kit and was evaluated in terms of quality and quantity. The study of polymorphisms of genesCDKN2A was performed using Polymerase Chain Reaction (PCR) and sequencing. According to thisresearch which is conducted on the profiles within the years of 2007 to 2015 in the north of thecountry, non-melanoma cancer is seen in 100% of the cases in which the ratio of Squmous cellcarcinoma (SCC) is more than Basal cell carcinoma (BCC). In order to study intron areapolymorphism the samples are sequenced. With respect to all sequences, no polymorphism isobserved in rs36168473 in intron (1-2) of gene CDKN2A. Similar studied on the same gene and thesame type of cancer with other RSs in different directions has shown a polymorphism withalternating frequency. But given that this rs36168473 is new and selected from gene intron area, nopolymorphism is observed in this area.کلیدواژه ها
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