SiRNA Knock-Down Of HIF-1α Resulted In Dramatically Changes In Cell Cycle And Viability Of U87 Cell Line
- سال انتشار: 1395
- محل انتشار: دومین سمپوزیوم بین المللی سرطان نسترن
- کد COI اختصاصی: NASTARANCANSER02_065
- زبان مقاله: انگلیسی
- تعداد مشاهده: 445
نویسندگان
Department Of Genetics, Faculty Of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department Of Nanobiotechnology, Faculty Of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department Of Genetics, Faculty Of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department Of Genetics, Faculty Of Biological Sciences, Tarbiat Modares University, Tehran, Iran
چکیده
HIF-1 is a transcription factor, composed of HIF-1α and HIF-1β subunits and functions as a masterregulator of oxygen homeostasis in all metazoan species. Upon hormonal stimulation or in oxygendeficientmicro-environment, HIF-1α is activated by ROS through a complex signaling network.Some studies showed that overexpression of HIF-1α is a negative prognostic indicator in manyhuman cancers such as breast, cervix, oropharynx, ovary and uterus. Moreover, significantassociationsbetween HIF-1α overexpression and patient mortalityhave been shown in cancers of thebrain. Here we aimed that to evaluate the effect of selective gene silencing ofHIF-1α with specificshort interfering RNA (siRNA) on the cell cycle and viability of U87 cell line as a human primaryglioblastoma cell line. U87 cells culturedin DMEM with 10% FBS supplementedwith penicillin andstreptomycin. Transfer of a specific siRNA against HIF-1α was performed by Lipofectamine®2000.RT-PCR analysis was performed after 24, 48 and 72 hours posttransfection.Using flowcytometry test and MTT assay, cell cycle and viability of transfected cells were analyzed. Using RTPCRmethod, we showed a significant down-regulation of HIF-1α in 48 hours transfectedcells incomparison with un-transfected cells. Moreover, we found knock down of HIF-1α significantly affectthe cell cycle and survival of u87 cells. Our results highlight the importance of Hif-1a as a candidatetarget in the field of cancer therapy.کلیدواژه ها
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